Anti-VGLL3 Rabbit Polyclonal Antibody for WB and ELISA - A8MV65

Core Product Specifications and Parameters

Biological Background: VGLL3 Function and Localization

• VGLL3 (Transcription cofactor vestigial-like protein 3) is encoded by the VGLL3 gene and belongs to the vestigial-like family, with alternative splicing producing multiple isoforms.
• May function as a specific coactivator for mammalian TEFs (TEAD transcription factors), playing a key role in transcription regulation.
• The protein is localized in the nucleus, consistent with its role as a transcription cofactor.
• According to UniProt, VGLL3 is enriched in placenta, suggesting tissue-specific transcriptional regulatory functions.
• The protein can undergo ubiquitination (Ubl conjugation) and contains isopeptide bonds, as identified by proteomics; these post-translational modifications may regulate its stability or activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 71-180 of human VGLL3; this region may be conserved across isoforms, potentially enabling broad isoform recognition.
• For Western blot analysis, consider testing a range of antibody dilutions (e.g., 1:500–1:2000) and validate in appropriate sample systems, such as placental tissue lysates.
• Reported cross-reactivity with rat suggests potential utility in rodent models; however, independent validation in the relevant species and tissue is recommended.
• For ELISA applications, direct immobilization of the immunogen peptide can serve as a positive control; pairing with a detection antibody for sandwich ELISA requires careful optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibodies like this anti-VGLL3 polyclonal antibody for transcription cofactor studies. Beyond individual reagents, we supply antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your workflow from concept to clinic. Contact us for bulk quantities, conjugation services, or technical consultation to accelerate your assay development.