Anti-Versican Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P13611

Core Product Specifications and Parameters

Biological Background: Versican Function and Localization

• Versican is a large chondroitin sulfate proteoglycan (also known as CSPG2) that mediates intercellular signaling, connects cells to the extracellular matrix, and regulates cell motility, growth, and differentiation.
• It binds hyaluronic acid with high affinity, contributing to matrix organization and hydration, and influencing processes such as tissue morphogenesis and repair.
• Alternative splicing produces four major isoforms (V0, V1, V2, V3) with tissue-specific expression: V0 and V1 are broadly expressed in brain and various tumors, V2 is restricted to normal brain and gliomas, and V3 is absent only from medulloblastomas. Related references: PMID:8627343
• Secreted into the extracellular space, versican localizes to specialized matrices such as the interphotoreceptor matrix, photoreceptor outer segments, and cilia, underscoring roles in sensory and structural biology.
• Versican is detected in placenta (PubMed:32337544), cerebrospinal fluid, fibroblasts, and urine (PubMed:25326458; PubMed:36213313), retina (PubMed:29777959), and cerebral white matter (PubMed:2469524), reflecting its broad yet compartmentalized distribution. Related references: PMID:32337544 PMID:25326458 PMID:36213313 PMID:29777959 PMID:2469524
• Post-translational features include glycosylation, phosphorylation, and disulfide bonds; domain motifs (EGF-like, immunoglobulin, Sushi, C-type lectin) and calcium binding implicate versican in diverse molecular interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1400–1586 within the C‑terminal G3 domain; verify detection of all relevant isoforms in your experimental system by parallel analysis of recombinant standards or isoform-specific controls.
• For Western blot, use low‑percentage polyacrylamide gels (4–6%) and extended wet transfer to accommodate the ~373 kDa full‑length protein; chondroitinase ABC treatment can aid in resolving core protein size by removing glycosaminoglycan chains.
• In immunofluorescence on paraffin‑embedded (IF‑P) or cell samples, optimize fixation and consider mild permeabilization to preserve extracellular matrix architecture; include an appropriate negative control.
• When developing ELISA assays, pair with a matched detection antibody and validate against recombinant versican or tissue lysates to confirm sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, bridging research and diagnostic development with a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents. Our versican polyclonal antibody supports investigations into extracellular matrix biology, cancer, and tissue remodeling—accelerate your assay pipelines by sourcing high‑quality raw materials from a single, trusted platform.