Anti-Versican Monoclonal Antibody for WB, ELISA - P13611

Core Product Specifications and Parameters

Biological Background: Versican Function and Localization

• Versican is a large chondroitin sulfate proteoglycan that belongs to the aggrecan/versican family. It is also known as CSPG2, PG-M, and glial hyaluronate-binding protein (GHAP).
• It plays a key role in intercellular signaling and connecting cells with the extracellular matrix, and binds hyaluronic acid, contributing to tissue hydration and structural integrity.
• Multiple isoforms are generated by alternative splicing (V0, V1, V2, V3), which differ in the number of chondroitin sulfate attachment domains and exhibit tissue-specific expression patterns.
• Versican is localized to the extracellular matrix and is secreted; it is also found in cell projections, including the photoreceptor outer segment and interphotoreceptor matrix in the retina.
• The protein undergoes extensive post-translational modifications, including glycosylation, phosphorylation, and formation of disulfide bonds. It contains EGF-like, immunoglobulin-like, and lectin domains, as well as sushi repeats.
• Tissue expression is broad, with detection in placenta, cerebrospinal fluid, fibroblasts, urine, retina, and brain. Isoform V0/V1 are expressed in various neural tissues and tumors, while V2 is restricted to normal brain and gliomas.
• Keywords associated with versican include alternative splicing, calcium binding, cataract, and extracellular matrix, indicating involvement in both normal physiology and disease conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 3200–3396 within the C-terminal region of human versican; this region may be present in multiple isoforms. Confirm specificity for the isoform of interest using appropriate controls.
• For Western blot analysis, due to the large predicted molecular weight (~373 kDa), consider using low-percentage polyacrylamide gels and extended transfer times to ensure efficient blotting.
• Validate antibody performance in relevant sample systems, such as human placenta or brain lysates, which are known to express versican. Mouse samples can also be used due to the antibody's cross-reactivity.
• For ELISA applications, optimize antibody concentration and use a suitable recombinant protein or peptide standard to generate a reliable standard curve.
• This antibody has not been tested in other applications; if attempting immunohistochemistry or immunofluorescence, note that versican is a secreted matrix protein and may require antigen retrieval or special fixation protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this anti-versican clone. For projects involving extracellular matrix biology, cell signaling, or proteoglycan studies, we offer one-stop access to optimized antibody pairs, bulk ancillary reagents, and sourcing of rare targets—supporting your workflow from concept to clinic.