Anti-VEPH1 Rabbit Polyclonal Antibody for WB, ELISA - Q14D04

Core Product Specifications and Parameters

Biological Background: VEPH1 Function and Localization

• VEPH1 (Ventricular zone-expressed PH domain-containing protein homolog 1), also known as Protein melted, is encoded by the VEPH1 gene (KIAA1692) in human.
• It functions as an inhibitor of TGF-beta signaling by interacting with TGF-beta receptor type-1 (TGFBR1) and impeding the dissociation of activated SMAD2, preventing its nuclear accumulation.
• May also impact FOXO, Hippo, and Wnt signaling pathways, suggesting broader regulatory roles.
• Localizes to the cell membrane.
• The protein undergoes alternative splicing, and is classified as a reference proteome component identified by proteomics.
• The calculated molecular weight is approximately 94.7 kDa, while the detected protein weight is 95 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 448-547 of human VEPH1 (NP_078897.2). For Western blot (WB), a band at approximately 95 kDa is expected; pre-validation in relevant sample systems (e.g., human or mouse cell lines) is recommended to confirm specificity and minimize background.
• For ELISA, consider direct or sandwich assay formats, and titrate the antibody to determine optimal concentration for antigen detection.
• Since VEPH1 is expressed at the cell membrane, consider subcellular fractionation to enrich membrane proteins for enhanced detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing diagnostic manufacturers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-VEPH1 antibody supports research into TGF-beta and related signaling pathways, and we can assist in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.