Anti-VEGFR3/FLT4 Rabbit Polyclonal Antibody for WB and ELISA - P35916

Core Product Specifications and Parameters

Biological Background: VEGFR3/FLT4 Function and Localization

• Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT4, is a tyrosine-protein kinase receptor that binds VEGFC and VEGFD.
• It plays an essential role in adult lymphangiogenesis and embryonic cardiovascular development, promoting endothelial cell proliferation, survival, and migration.
• Upon ligand binding, VEGFR3 activates downstream signaling pathways including MAPK/ERK, JUN, and AKT1, and phosphorylates SHC1 and PIK3R1.
• Alternative splicing generates isoforms; isoform 2 has a truncated C-terminus with reduced signaling, while secreted isoform 3 may act as a decoy receptor.
• Subcellularly, VEGFR3 is found at the cell membrane, in the cytoplasm, nucleus, and can be secreted.
• It is widely expressed, with protein detected in endothelial cells and tissues such as liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
• Keywords: ATP-binding, angiogenesis, kinase, transmembrane, phosphoprotein, receptor, and more.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1230-1329) is located in the cytoplasmic region of human VEGFR3; consider this when selecting detection methods.
• For Western blot, the expected band is approximately 153 kDa; using appropriate membrane and blocking conditions is recommended.
• In ELISA, use the antibody as per standard indirect detection protocols; validate in human sample systems.
• Since the antibody cross-reacts with human VEGFR3, species cross-reactivity for other species should be independently tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For VEGFR3/FLT4-targeted research in lymphangiogenesis and cancer, we offer a range of validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our comprehensive supply chain supports your assay development needs efficiently.