Anti-VEGFR2/CD309 Monoclonal Antibody for FC - P35968

Core Product Specifications and Parameters

Biological Background: VEGFR2/CD309 Function and Localization

• VEGFR2 (KDR/FLK1) is a tyrosine-protein kinase receptor for VEGFA, VEGFC, and VEGFD, essential for angiogenesis, vascular development, and endothelial cell function.
• Isoforms lacking the transmembrane domain (isoform 2 and 3) act as decoy receptors, negatively regulating lymphangiogenesis by sequestering VEGFA/VEGFC.
• Mediates multiple signaling cascades including MAPK/ERK, AKT1, and PLCG1 pathways, promoting endothelial cell proliferation, survival, migration, and cytoskeletal reorganization.
• Plays a role in embryonic hematopoiesis and modulates FLT1 and FLT4 signaling through heterodimerization.
• Subcellular localization includes cell membrane, cytoplasm, nucleus, cell junctions, endoplasmic reticulum, and early endosomes; also found secreted.
• Detected in cornea (at protein level) and widely expressed across tissues.
• Post-translational modifications include phosphorylation, glycosylation, and ubiquitination; contains immunoglobulin domains and disulfide bonds.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen targets the extracellular domain of human VEGFR2, suitable for detecting cell surface expression in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to assess specificity and exclude dead cells.
• is a bright and photostable fluorophore, enabling sensitive detection and compatibility with multicolor panel designs due to minimal spectral spillover.
• Validate the antibody’s reactivity in human endothelial cell lines or relevant primary cells, as cross-reactivity is only confirmed for human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, covering the entire development journey from concept to clinic. This anti-VEGFR2/CD309 antibody supports angiogenesis and vascular biology research, and our portfolio includes validated monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents for oncology and biomarker applications. Partner with CamelBio for efficient sourcing and tailored support to accelerate your diagnostic development.