Anti-VEGFR1/FLT-1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17948

Core Product Specifications and Parameters

Biological Background: VEGFR1 Function and Localization

• VEGFR1, encoded by the FLT1 gene (also known as FLT, FRT), is a receptor tyrosine kinase that serves as a high-affinity receptor for vascular endothelial growth factor A (VEGFA), as well as VEGFB and placental growth factor (PGF).
• It plays a crucial role in embryonic vasculature development, angiogenesis, endothelial cell survival, migration, macrophage function, and cancer cell invasion.
• The receptor is located on the cell membrane and can be internalized into endosomes; alternative splicing also generates secreted and cytoplasmic isoforms.
• Tissue-specific expression includes placenta (isoform 2), corneal epithelial cells and vascular smooth muscle cells (isoform 3), and wide expression in vascular endothelial cells and peripheral blood monocytes.
• Ligand binding triggers activation of downstream signaling cascades. VEGFR1 mediates phosphorylation of PIK3R1, MAPK1/ERK2, MAPK3/ERK1, AKT1 at 'Ser-473', SRC, YES1, and PTK2/FAK1. Related references: PMID:16685275
• VEGFR1 may also function as a decoy receptor to negatively regulate VEGFA signaling by sequestering VEGFA and preventing its binding to KDR.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination.
• The full-length protein has a predicted molecular weight of approximately 151 kDa (UniProt: ~150.8 kDa).

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the C-terminal region (aa 1100-1180) of human VEGFR1, which is part of the intracellular kinase domain. This design may facilitate detection of multiple isoforms containing this region.
• For Western blot (WB), a band around 151 kDa is expected for the full-length receptor. Reduced migration or additional bands may reflect glycosylation or alternative splicing variants; validate in the relevant sample system.
• For immunofluorescence (IF/ICC), permeabilization of cells is required to access the intracellular epitope. Consider using appropriate controls to confirm specificity.
• ELISA applications can be optimized by determining the optimal coating concentration and using validated detection antibodies. Cross-reactivity with mouse and rat VEGFR1 suggests suitability for orthologous protein detection in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers, research labs, and institutes. With this anti-VEGFR1 polyclonal antibody and a portfolio of validated antibody pairs, optimized monoclonals/polyclonals, and ancillary reagents, we support angiogenesis- and oncology-focused assay development. Our sourcing service extends to rare target raw materials, ensuring a seamless bridge from concept to clinic.