Anti-VEGFR1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17948

Core Product Specifications and Parameters

Biological Background: VEGFR1 Function and Localization

• VEGFR1 (FLT-1) is a tyrosine-protein kinase receptor and member of the VEGFR family, encoded by the FLT1 gene. It exists as multiple isoforms, including a membrane-bound full-length form and soluble secreted variants (isoforms 2 and 3) generated by alternative splicing.
• Acts as a high-affinity receptor for VEGFA, VEGFB and PGF, critically regulating embryonic vasculature development and postnatal angiogenesis. It can both promote endothelial cell survival and proliferation, and function as a decoy receptor to negatively modulate VEGFA signaling by sequestering free ligand.
• Ligand binding activates multiple downstream pathways, including PLCG-PKC, PI3K-AKT1, and MAPK signaling cascades. It phosphorylates key mediators such as SRC, YES1, CBL, and PTK2/FAK1, and induces AKT1 phosphorylation at Ser-473. Related references: PMID:16685275
• Subcellularly, VEGFR1 is predominantly localized at the plasma membrane but also detected in endosomes, cytoplasm, and as secreted isoforms, reflecting its dynamic trafficking and signaling versatility.
• It is widely expressed in vascular endothelial cells and tissues such as placenta, liver, kidney, heart, and brain. Isoform 2 is abundant in placenta, while isoform 3 is found in corneal epithelial cells and vascular smooth muscle cells.
• The protein undergoes extensive post-translational modifications, including N-linked glycosylation, disulfide bond formation, and phosphorylation, which are critical for its function. UniProt keywords highlight its roles in angiogenesis, chemotaxis, and development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 20-300) of human VEGFR1, suggesting reactivity with both membrane-bound and soluble isoforms. Perform appropriate epitope validation in your experimental system.
• For Western blotting, the expected molecular weight is approximately 151 kDa; however, glycosylation may cause band shifts. Validate specificity using appropriate controls (e.g., blocking peptide or knock-down lysates).
• In immunohistochemistry (IHC-P), robust antigen retrieval methods are recommended for formalin-fixed paraffin-embedded tissues. Begin with standard citrate or EDTA-based protocols and optimize detection conditions.
• For ELISA, the antibody can be used as a capture or detection reagent. Titrate the antibody to determine optimal working concentrations, and cross-validate with complementary antibody pairs for sandwich formats.
• Given cross-reactivity with mouse and rat, the antibody is suitable for translational studies but confirm species-specific performance with relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including this VEGFR1 polyclonal antibody, supporting angiogenesis and cancer research. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents—all from a single, reliable source. Whether you need bulk quantities or specialized rare-material sourcing, CamelBio bridges your diagnostic development from concept to clinic.