Anti-VEGFR1 Rabbit Monoclonal Antibody for WB, ELISA - P17948

Core Product Specifications and Parameters

Biological Background: VEGFR1 Function and Localization

• VEGFR1 (FLT-1) is a tyrosine-protein kinase receptor for VEGFA, VEGFB, and PGF, essential for embryonic vasculature development and regulation of angiogenesis, cell migration, and survival.
• Acts as both a positive regulator (promotes endothelial proliferation and angiogenesis in adulthood) and a negative regulator (limits excessive endothelial proliferation during embryogenesis).
• Functions as a decoy receptor for VEGFA, sequestering free VEGFA to modulate signaling through KDR/VEGFR2; possesses very high ligand affinity but low kinase activity.
• Ligand binding triggers multiple signaling cascades: PLCG leads to PKC activation; PI3K-AKT pathway; MAPK/ERK pathway. Phosphorylates SRC, YES1, and PTK2/FAK1. Related references: PMID:16685275
• Subcellular localization includes cell membrane, endosome, secreted forms, and cytoplasm; isoforms exhibit distinct distributions.
• Isoforms show tissue-specific expression: isoform 2 strongly in placenta, isoform 3 in corneal epithelial cells and vascular smooth muscle cells; broadly detected in lung, liver, kidney, heart, and brain.
• Keywords: alternative splicing, angiogenesis, ATP-binding, glycoprotein, immunoglobulin domain, kinase, transmembrane, tyrosine-protein kinase.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), likely ensuring specific targeting of the extracellular domain of VEGFR1.
• Designed for western blot (WB) and ELISA; given the high molecular weight (~151 kDa), consider adequate protein transfer conditions and use appropriate protein markers.
• Cross-reactivity with mouse and rat is reported, enabling utility in common rodent models for angiogenesis and cancer studies.
• As with any antibody, empirical optimization of dilution and buffer conditions is recommended; validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and laboratories with validated antibodies, optimized recombinant proteins, and ancillary reagents. This VEGFR1 monoclonal antibody supports angiogenesis and cancer research —and we can supply bulk quantities, matched antibody pairs, and rare target raw materials to streamline your assay development from concept to clinic.