Anti-VEGFC Rabbit Polyclonal Antibody for WB, ELISA - P49767

Core Product Specifications and Parameters

Biological Background: VEGFC Function and Localization

• Vascular endothelial growth factor C (VEGFC), also known as Flt4 ligand (Flt4-L) and vascular endothelial growth factor-related protein (VRP), is a member of the VEGF family.
• It functions as a growth factor active in angiogenesis and endothelial cell growth, stimulating proliferation and migration, and also affecting blood vessel permeability.
• VEGFC plays a critical role in lymphangiogenesis, contributing to the development of venous and lymphatic vascular systems during embryogenesis and the maintenance of differentiated lymphatic endothelium in adults.
• It exerts its effects by binding and activating the receptor tyrosine kinases KDR/VEGFR2 and FLT4/VEGFR3.
• The protein is secreted and undergoes post-translational modifications including glycosylation, disulfide bond formation, and cleavage on pairs of basic residues to generate mature forms.
• VEGFC is expressed in a wide range of tissues including spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, skeletal muscle, ovary, small intestine, prostate, testis, and colon. Related references: PMID:8700872, PMID:9247316, PMID:8617204
• Keywords associated with VEGFC include angiogenesis, developmental protein, differentiation, mitogen, growth factor, and secreted, reflecting its multifaceted roles in vascular biology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 200-300 of human VEGFC. When using this antibody in WB, consider detecting a band at approximately 47 kDa under reducing conditions. Validate the signal using positive controls such as endothelial cell lysates or tissues known to express VEGFC.
• For ELISA applications, optimize the coating antigen concentration and detection antibody to achieve desired sensitivity. Include appropriate negative controls (e.g., antigen-free wells) to assess background.
• As a polyclonal antibody, there may be lot-to-lot variability in affinity and specificity. It is advisable to test each new lot in the specific experimental context and consider cross-reactivity with other VEGF family members if relevant to your study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of validated antibodies, antibody pairs, and bulk ancillary reagents. For angiogenesis and lymphangiogenesis research, we can supply anti-VEGFC polyclonal antibodies like this product, along with optimized monoclonal antibodies and rare-target raw materials to support your diagnostic and research workflows. Let us help you bridge the gap from concept to clinic with reliable, high-quality reagents.