Anti-VEGFC (Flt4-L, VRP) Rabbit Monoclonal Antibody for WB and ELISA - P49767

Core Product Specifications and Parameters

Biological Background: VEGFC Function and Localization

• Vascular endothelial growth factor C (VEGFC/Flt4-L/VRP) is a secreted member of the VEGF family, encoded by the VEGFC gene (GeneID: 7424).
• It functions as a critical regulator of angiogenesis and lymphangiogenesis, promoting endothelial cell proliferation, migration, and vascular permeability.
• VEGFC binds and activates the receptor tyrosine kinases VEGFR2 (KDR) and VEGFR3 (FLT4), thereby stimulating signaling in both blood vascular and lymphatic endothelial cells.
• During embryogenesis, VEGFC is essential for the development of the venous and lymphatic vascular systems; in adults, it maintains differentiated lymphatic endothelium.
• VEGFC is expressed in a variety of tissues, including spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, skeletal muscle, ovary, small intestine, prostate, testis, and colon. Related references: PMID:8700872, PMID:9247316, PMID:8617204
• Post-translational modifications, such as cleavage at paired basic residues, glycosylation, and disulfide bond formation, are critical for its activation and receptor interaction.
• The mature protein has a predicted molecular weight of approximately 47 kDa (full-length preproprotein), with processing generating multiple secreted forms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 112–227 of human VEGFC (NP_005420.1). This region may be conserved across species, though cross-reactivity is reported for human only; verify in your sample system.
• For Western blotting, a band at ~47 kDa (full-length) and potentially smaller processed forms may be observed. Use reducing conditions and validate with appropriate positive controls (e.g., tissues with known VEGFC expression).
• In ELISA, this antibody can be used for antigen capture or detection; consider testing in direct or sandwich formats with recombinant VEGFC protein.
• Because VEGFC is a secreted factor, ensure sample preparation protocols (e.g., serum, conditioned medium) are optimized to preserve native protein levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic developers and research labs to deliver high-quality IVD raw materials for angiogenesis and lymphangiogenesis research. From validated anti-VEGFC monoclonal antibodies to bulk ancillary reagents, we provide reliable, traceable supplies to accelerate your assay development. With end-to-end consulting and technical support, CamelBio simplifies raw-material procurement so you can focus on translating science into clinical solutions.