Anti-VEGFB Polyclonal Antibody for WB, IF/ICC, ELISA - P49765

Core Product Specifications and Parameters

Biological Background: VEGFB Function and Localization

• Vascular endothelial growth factor B (VEGFB) is a secreted growth factor that promotes endothelial cell proliferation and survival, playing a key role in angiogenesis and vascular homeostasis.
• VEGFB exists as two major isoforms, VEGF-B167 and VEGF-B186, generated by alternative splicing; both isoforms share a common N-terminal region but differ in their C-terminal domains.
• VEGF-B167 binds to heparin and neuropilin-1 with high affinity, while the neuropilin-1 binding of VEGF-B186 is regulated by proteolytic processing.
• The protein is classified as a glycoprotein with disulfide bonds, characteristic of the VEGF/PDGF family of growth factors, and functions as a mitogen for endothelial cells.
• Subcellularly, VEGFB is secreted into the extracellular space, where it can interact with receptors on target cells to activate downstream signaling pathways.
• Tissue expression analysis reveals that VEGFB is broadly expressed, with highest levels in heart, skeletal muscle, and pancreas; it is absent in the liver.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody covers amino acids 30-121 of human VEGFB, a region well-conserved between the two isoforms and across species, which may contribute to the observed cross-reactivity with mouse and rat.
• For Western blot (WB), consider using a reducing sample buffer and detect a band at approximately 22 kDa, corresponding to the mature monomeric form; however, dimers or glycosylated variants may also be observed.
• Immunofluorescence (IF/ICC) may require optimization of fixation and permeabilization conditions, as VEGFB is a secreted protein; incubation with a protein transport inhibitor may enhance intracellular staining.
• ELISA performance should be validated with appropriate recombinant VEGFB standards and sample matrices, as the antibody is a polyclonal preparation and may exhibit lot-to-lot variability.
• Always titrate the antibody in your specific experimental system to determine the optimal working dilution, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. With a focus on cardiovascular and angiogenesis-related targets like VEGFB, our portfolio supports the development of robust immunoassays and research tools. We provide one-stop access to technical services and consulting to accelerate your project from concept to clinical application.