Anti-VEGFA Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15692

Core Product Specifications and Parameters

Biological Background: VEGFA Function and Localization

• VEGFA participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis, such as HIF1A. Related references: PMID:35455969
• VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Related references: PMID:34530889
• VEGFA binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, as well as NRP1/neuropilin-1. One isoform binds the KDR receptor but does not activate downstream signaling pathways and inhibits tumor growth.
• The protein localizes to multiple subcellular compartments, including the cytoplasm, nucleus, endoplasmic reticulum, Golgi apparatus, and is secreted into the extracellular space and matrix.
• VEGFA is a glycoprotein with heparin-binding affinity and contains disulfide bonds, which are essential for its structural integrity and function.
• VEGFA is widely expressed, with higher expression in pituitary tumors compared to the normal pituitary gland. Certain isoforms exhibit restricted expression patterns.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-200 of human VEGFA (NP_001165094.1). Due to alternative splicing and multiple isoforms, consider confirming detection of the specific isoform(s) relevant to your study through sequence alignment.
• For Western blot, the predicted molecular weight is approximately 27 kDa; however, glycosylation and potential isoform variations may result in additional bands. Use appropriate positive controls and molecular weight markers.
• In IHC-P and IF/ICC, antigen retrieval and permeabilization steps may be necessary to access VEGFA in its various subcellular locations. Optimize detection conditions for your tissue or cell type.
• For ELISA applications, as a polyclonal antibody, it may provide high sensitivity but could exhibit broader specificity. In quantitative assays, validate with standard curves and consider using it as a capture or detection reagent in a matched pair system.
• Cross-reactivity is reported for human, mouse, and rat; however, always validate in the specific species and sample system of interest, as epitope conservation and expression levels can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing platform for IVD raw materials. This anti-VEGFA polyclonal antibody supports critical investigations into angiogenesis, hypoxia, and tumor biology. From validated antibody pairs and recombinant proteins to bulk ancillary reagents and rare target sourcing, we help accelerate your assay development from concept to clinic.