Anti-VEGFA Polyclonal Antibody for WB, IF/ICC, ELISA - P15692

Core Product Specifications and Parameters

Biological Background: VEGFA Function and Localization

• VEGFA (Vascular endothelial growth factor A) is a long-form growth factor that plays a central role in angiogenesis, vasculogenesis, and endothelial cell proliferation, migration, and survival. It binds to receptors FLT1/VEGFR1 and KDR/VEGFR2, as well as NRP1 and DEAR/FBXW7-AS1, to mediate diverse signaling pathways. Related references: PMID:34530889 PMID:17446437
• VEGFA participates in the hypoxic response by inducing key genes such as HIF1A, and it protects cells from hypoxia-mediated cell death. Related references: PMID:35455969
• A specific isoform of VEGFA binds to KDR but fails to activate downstream signaling, thereby inhibiting angiogenesis and suppressing tumor growth.
• The protein is found in multiple subcellular locations, including secreted forms in the extracellular space and matrix, as well as intracellular pools in the cytoplasm, nucleus, endoplasmic reticulum, and Golgi apparatus.
• VEGFA is widely expressed in many tissues, with notably higher expression levels observed in pituitary tumors compared to normal pituitary gland.
• The protein features a heparin-binding domain, undergoes disulfide bond formation and glycosylation, and functions as a mitogen and developmental factor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 91–191 of human VEGFA, a sequence found in multiple isoforms; thus, the polyclonal antibody may recognize various splice variants.
• For western blotting, a band around 27 kDa is reported under reducing conditions, but molecular weight may shift due to glycosylation or isoform differences; loading controls and knockout/knockdown validation are advised.
• In immunofluorescence/immunocytochemistry, both secreted and intracellular VEGFA can be detected; optimize fixation and permeabilization protocols to preserve epitopes.
• For ELISA applications, the antibody can be considered for capture or detection in sandwich formats, but pairing with a compatible antibody and thorough validation is necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For angiogenesis or hypoxia-related target development, CamelBio offers a full portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing to accelerate your IVD assay development. Partner with CamelBio to streamline your workflow and secure reliable, high-quality raw materials for VEGFA and beyond.