Anti-VEGFA Mouse Monoclonal Antibody for WB, ELISA, IHC-P - P15692

Core Product Specifications and Parameters

Biological Background: VEGFA Function and Localization

• Vascular endothelial growth factor A (VEGFA), also designated vascular permeability factor (VPF), is a member of the PDGF/VEGF growth factor family. Multiple isoforms are produced through alternative splicing, alternative initiation, and alternative promoter usage.
• It acts as a key regulator of angiogenesis and vasculogenesis, stimulating endothelial cell proliferation, migration, and survival while increasing vascular permeability (PubMed:34530889). Related references: PMID:34530889
• VEGFA binds to tyrosine kinase receptors FLT1/VEGFR1 and KDR/VEGFR2, as well as neuropilin-1 (NRP1). An isoform that binds KDR without activating downstream signaling inhibits angiogenesis and tumor growth.
• It participates in the cellular response to hypoxia by inducing the expression of HIF1A and other hypoxia-regulated genes, thereby protecting cells from hypoxia-induced apoptosis (PubMed:35455969). Related references: PMID:35455969
• The protein localizes to multiple compartments: secreted forms are found in the extracellular space and matrix, while intracellularly it is detected in the cytoplasm, nucleus, endoplasmic reticulum, and Golgi apparatus, reflecting its synthesis and secretion pathway.
• Widely expressed in many tissues, with elevated expression observed in pituitary tumors compared to the normal pituitary gland.
• Post-translational modifications include N-linked glycosylation and intramolecular disulfide bonds, which are critical for proper folding and receptor binding.
• Keywords associated with VEGFA: angiogenesis, growth factor, mitogen, heparin-binding, developmental protein, differentiation, glycoprotein, secreted, disulfide bond, alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 207–327 of human VEGFA (P15692). For Western blot, a band around 27 kDa may be observed; confirm in your experimental system.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody titration should be optimized using VEGFA-positive tissue sections or cell pellets.
• The antibody cross-reacts with mouse and rat VEGFA; validate performance in the relevant species and application by including appropriate positive and negative controls.
• For ELISA, consider using recombinant VEGFA as a standard and testing serial dilutions to establish the linear detection range.
• As a mouse monoclonal antibody, it may require an anti-mouse secondary antibody for detection; ensure compatibility with the detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. For VEGFA-related diagnostic development, we provide one-stop access to optimized antibody pairs, bulk reagent sourcing, and custom procurement for rare targets. From concept to clinic, CamelBio streamlines your raw-material supply chain, enabling efficient assay development for angiogenesis and cancer biomarker applications.