Anti-VEGF Receptor 2 Polyclonal Antibody for WB, ELISA - P35968

Core Product Specifications and Parameters

Biological Background: VEGF Receptor 2 Function and Localization

• VEGF Receptor 2 (KDR/FLK-1) is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC, and VEGFD, playing an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.
• Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD, thereby negatively regulating lymphangiogenesis.
• The protein is localized across multiple subcellular compartments including the cell membrane, cytoplasm, nucleus, endoplasmic reticulum, early endosomes, and is also secreted.
• Mediates activation of key signaling pathways such as MAPK/ERK, AKT1, and PLCG1, promoting endothelial cell proliferation, survival, migration, and reorganization of the actin cytoskeleton.
• Post-translational modifications include phosphorylation, ubiquitination (Ubl conjugation), and glycosylation; it is annotated as a glycoprotein and phosphoprotein.
• Widely expressed in tissues, with specific detection in cornea at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (aa 1147-1356), enabling detection of total receptor protein regardless of extracellular domain shedding; confirm specificity in the relevant sample system.
• For Western blot, consider using reducing conditions and validate the antibody in endothelial cell lines (e.g., HUVEC) known to express VEGFR2.
• For ELISA, consider coating with recombinant VEGFR2 protein and optimizing detection conditions; cross-reactivity with mouse and rat allows broader model organism studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with cost-effective, one-stop access to IVD raw materials. For angiogenesis and vascular biology programs, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials. Whether you need this polyclonal antibody for routine screening or require custom antibody development, CamelBio bridges the gap from concept to clinic with reliable supply and technical support.