Anti-VEGF Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P15692

Core Product Specifications and Parameters

Biological Background: VEGF Function and Localization

• VEGF is a key growth factor that induces angiogenesis, vasculogenesis, and endothelial cell proliferation, migration, and survival; it also increases vascular permeability (PubMed:34530889). It binds to receptors FLT1/VEGFR1, KDR/VEGFR2, heparan sulfate, heparin, and NRP1/neuropilin-1 (By similarity), as well as the DEAR/FBXW7-AS1 receptor (PubMed:17446437). Related references: PMID:34530889 PMID:17446437
• VEGF plays a critical role in the cellular response to hypoxia by inducing HIF1A and protecting cells from hypoxia-mediated cell death (PubMed:35455969; By similarity). Related references: PMID:35455969
• An isoform of VEGF binds KDR but does not activate downstream signaling, thereby inhibiting angiogenesis and suppressing tumor growth.
• The protein localizes to the cytoplasm and nucleus, and is secreted into the extracellular space, including the endoplasmic reticulum, Golgi, and extracellular matrix.
• VEGF is widely expressed, with higher levels observed in pituitary tumors compared to normal pituitary gland.
• The gene undergoes alternative splicing and alternative promoter usage, producing multiple isoforms with distinct functions; post-translational modifications include glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• For Western blotting, consider running samples under both reducing and non-reducing conditions to observe monomeric (~15-27 kDa) and dimeric (~34-45 kDa) VEGF forms; the product may detect bands corresponding to these molecular weights.
• Validate antibody specificity and cross-reactivity in the relevant species (human, mouse, rat) and application system by including appropriate controls.
• In immunofluorescence/ICC, optimize permeabilization protocols to detect intracellular VEGF pools.
• For ELISA, pair this antibody with a suitable capture or detection reagent and confirm performance with recombinant VEGF standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supplying high-quality antibodies, antigen pairs, and ancillary reagents from research to manufacturing. With this anti-VEGF monoclonal antibody, we support angiogenesis and hypoxia research—and can source validated antibody pairs, optimized monoclonal/polyclonal formats, bulk reagents, and even rare-target raw materials to accelerate your diagnostic development.