Anti-VEGF-D Rabbit Polyclonal Antibody for WB and ELISA - O43915

Core Product Specifications and Parameters

Biological Background: VEGF-D Function and Localization

• VEGF-D (Vascular endothelial growth factor D), also known as c-Fos-induced growth factor (FIGF), is a secreted glycoprotein belonging to the PDGF/VEGF growth factor family.
• It functions as a growth factor active in angiogenesis, lymphangiogenesis, and endothelial cell growth by stimulating proliferation and migration, and also affects blood vessel permeability.
• During embryogenesis, VEGF-D may participate in the formation of the venous and lymphatic vascular systems, and in adults it helps maintain differentiated lymphatic endothelium.
• VEGF-D exerts its effects by binding and activating the receptor tyrosine kinases VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4).
• The protein is proteolytically processed at pairs of basic residues to generate mature forms.
• Subcellular location: VEGF-D is secreted into the extracellular space.
• Tissue specificity: Highly expressed in lung, heart, small intestine, and fetal lung, with lower expression in skeletal muscle, colon, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 20–220 of human VEGF-D, which spans the conserved VEGF homology domain (excluding the signal peptide). This design may yield antibodies that recognize both full-length and proteolytically processed forms.
• For Western blot (WB), the predicted molecular weight is approximately 40 kDa, but glycosylation and dimerization can result in bands at higher molecular weights. Consider using reducing and non-reducing conditions and validate with appropriate positive control lysates (e.g., lung or heart tissue).
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. It is advisable to validate the assay with recombinant human VEGF-D and native samples.
• As a rabbit polyclonal, it offers multi-epitope recognition, which may enhance sensitivity in detection assays but requires careful lot-to-lot consistency evaluation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like VEGF-D involved in angiogenesis and lymphangiogenesis, we facilitate one-stop sourcing of rare and specialized reagents to accelerate your assay development and validation. Partner with CamelBio to streamline your supply chain and bring reliable diagnostic solutions from concept to clinic.