Anti-VE-Cadherin Rabbit PolymAb® Monoclonal Antibody for WB, IHC-P, ELISA - P33151 / P55284

Core Product Specifications and Parameters

Biological Background: VE-Cadherin Function and Localization

• VE-cadherin (Cadherin-5) is a calcium-dependent cell adhesion protein belonging to the cadherin family, also known as 7B4 antigen or CD144, encoded by the CDH5 gene in humans.
• It mediates homophilic cell-cell adhesion, contributing to the sorting of heterogeneous cell types. Related references: PMID:21269602
• It plays a key role in endothelial cell cohesion and intercellular junction organization.
• Through association with α-catenin, VE-cadherin links to the actin cytoskeleton, and acts as a junctional anchor for AMOTL2 and MAGI1 to couple actin fibers to endothelial cell junctions. Related references: PMID:10861224
• It collaborates with KRIT1 and PALS1 to establish and maintain endothelial cell polarity and vascular lumen formation, mediated by recruitment of the Par polarity complex and RAP1B. Related references: PMID:20332120
• VE-cadherin positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to mechanical cues (mechanotransduction). Related references: PMID:25795300
• Subcellularly, it localizes predominantly at cell junctions (adherens junctions), the cell membrane, and in the cytoplasm.
• Tissue expression is high in endothelial cells and also found in the brain. Related references: PMID:27338829, PMID:2059658

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 259–358 within the extracellular cadherin repeat region, conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, expect a band near 88 kDa; use endothelial cell lysates (e.g., HUVEC) as positive control and validate with appropriate loading controls.
• For IHC-P, optimize antigen retrieval conditions; vascular endothelium serves as a suitable positive control tissue.
• In ELISA, this antibody can be used for capture or detection; sandwich assays benefit from pairing with a clone targeting a non-overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, providing diagnostic manufacturers and researchers with premium reagents such as this anti-VE-cadherin antibody for vascular biology applications. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development. Contact our team to learn how we can support your project from concept to clinic.