Anti-VE Cadherin Rabbit Polyclonal Antibody for WB, ELISA - P33151

Core Product Specifications and Parameters

Biological Background: VE Cadherin Function and Localization

• VE Cadherin, also known as Cadherin-5, CD144, or 7B4 antigen, is a classical cadherin adhesion molecule encoded by the CDH5 gene.
• It functions as a calcium-dependent cell adhesion protein that mediates homophilic interactions, contributing to the sorting of heterogeneous cell types. Related references: PMID:21269602
• In endothelial cells, VE Cadherin associates with alpha-catenin to link the actin cytoskeleton to cell junctions, and anchors proteins such as AMOTL2 and MAGI1. Related references: PMID:10861224
• It collaborates with KRIT1 and PALS1 to regulate endothelial cell polarity and vascular lumen formation, involving the Par polarity complex and RAP1B. Related references: PMID:20332120
• Subcellularly, it is located at cell-cell adherens junctions, the cell membrane, and also found in the cytoplasm.
• Tissue-specific expression includes endothelial cells (at protein level) and the brain. Related references: PMID:27338829, PMID:2059658
• VE Cadherin positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to mechanical forces. Related references: PMID:25795300
• Post-translational modifications include phosphorylation and glycosylation; it contains calcium-binding repeats, undergoes alternative splicing, and is cleaved on pairs of basic residues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 259-358 of human VE Cadherin, a region likely conserved in mouse, supporting the observed cross-reactivity.
• For Western blot (WB), consider the predicted molecular weight of ~88 kDa when selecting gel percentage and transfer conditions; validate with appropriate controls.
• ELISA applications may require optimization of coating and detection conditions for optimal signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot variation is possible; we recommend validating performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering a comprehensive portfolio of antibodies, ancillary reagents, and technical consulting to support every stage from concept to clinic. Our VE Cadherin polyclonal antibody is part of our expanding endothelial cell adhesion marker collection, ideal for research and assay development. We also provide custom sourcing and bulk supply solutions to meet your diagnostic development needs.