Anti-VE Cadherin Polyclonal Antibody for WB, IHC-P, ELISA - P33151

Core Product Specifications and Parameters

Biological Background: VE Cadherin Function and Localization

• Cadherin-5, also known as VE-cadherin, 7B4 antigen, or CD144, encoded by the CDH5 gene, is a calcium-dependent cell adhesion protein.
• It mediates homophilic cell-cell interactions, contributing to the sorting of heterogeneous cell types (PubMed:21269602). Related references: PMID:21269602
• Plays a central role in endothelial cell biology by controlling the cohesion and organization of intercellular junctions; associates with alpha-catenin to link to the cytoskeleton (PubMed:10861224). Related references: PMID:10861224
• Works in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen, mediated by the Par polarity complex and RAP1B (PubMed:20332120). Related references: PMID:20332120
• Positively regulates reorientation of actin stress fibers and endothelial cell alignment in response to mechanotransduction (PubMed:25795300). Related references: PMID:25795300
• Controls cadherin-5 endocytosis through association with CTNND1/p120-catenin (By similarity).
• Localizes to cell junctions, adherens junctions, cell membrane, and cytoplasm.
• Expressed in endothelial cells (at protein level) and in the brain (PubMed:27338829, PubMed:2059658). Related references: PMID:27338829, PMID:2059658

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular region (aa 621–784) of human VE-cadherin; the antibody may recognize both full-length and truncated forms.
• For Western blot, consider using endothelial cell lysates (e.g., HUVEC) as positive control; the predicted molecular weight is approximately 88 kDa.
• In immunohistochemistry (IHC-P), verify staining specificity in vascular-rich tissues such as placenta or brain, and include appropriate negative controls.
• For ELISA, the antibody may be suitable for capture or detection, but pairing with another clone should be validated in the user’s system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive sourcing bridge for IVD raw materials. This anti-VE-cadherin polyclonal antibody supports studies in endothelial cell biology and vascular integrity. As part of our portfolio, we also offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.