Anti-VDR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11473

Core Product Specifications and Parameters

Biological Background: VDR Function and Localization

• VDR (Vitamin D3 receptor), also known as NR1I1 (nuclear receptor subfamily 1 group I member 1) and PPP1R163, is a member of the nuclear receptor family of transcription factors.
• It functions as the receptor for calcitriol (1,25-dihydroxyvitamin D3), the active form of vitamin D, mediating genomic actions upon ligand binding. Related references: PMID:10678179, PMID:15728261, PMID:16913708
• Upon vitamin D3 binding, VDR translocates to the nucleus and forms heterodimers with the retinoid X receptor (RXR) to regulate transcription of target genes. Related references: PMID:28698609
• VDR plays a central role in calcium homeostasis by controlling the expression of genes involved in calcium absorption and bone metabolism.
• Beyond its classical endocrine role, VDR also functions as a receptor for secondary bile acid lithocholic acid (LCA) and its metabolites, linking vitamin D signaling to bile acid detoxification. Related references: PMID:12016314, PMID:32354638
• The protein localizes to both the nucleus and cytoplasm, with nuclear translocation enhanced upon ligand activation.
• Key structural features include a DNA-binding domain with zinc-finger motifs and a ligand-binding domain, as indicated by keywords: zinc, zinc-finger, DNA-binding, metal-binding.
• Alternative splicing generates multiple isoforms, and the protein is subject to post-translational modifications including ubiquitin conjugation (Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the N-terminal region (amino acids 1–100) of human VDR, potentially recognizing both full-length and certain variant isoforms.
• For Western blot (WB), consider using whole-cell lysates from VDR-expressing tissues or cell lines; note the predicted molecular weight of 48 kDa.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization steps to detect both cytoplasmic and nuclear VDR signals.
• For ELISA applications, validate the antibody as a capture or detection reagent with recombinant VDR protein or peptide standards.
• Always include appropriate controls (e.g., VDR-overexpressing lysates, blocking peptide) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For VDR-related research and assay development, we offer a broad portfolio including validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to learn how our integrated supply solutions can streamline your vitamin D receptor studies and diagnostic kit production.