Anti-VDAC3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y277

Core Product Specifications and Parameters

Biological Background: VDAC3 Function and Localization

• VDAC3 is a voltage-dependent anion channel (VDAC) that localizes to the mitochondrial outer membrane and plasma membrane, mediating non-selective transport of ions and metabolites. Related references: PMID:31935282
• It forms a high-conducting channel with a stable open state and a mild anion preference, displaying voltage-induced closure typical of porin channels. Related references: PMID:31935282
• VDAC3 plays a critical role in male fertility and sperm mitochondrial sheath formation, as suggested by similarity studies.
• The protein is widely expressed, with highest levels in testis, and is also detected in erythrocytes. Related references: PMID:9781040, PMID:27641616
• VDAC3 contains multiple phosphorylation, acetylation, and ubiquitination sites, indicating complex post-translational regulation.
• It belongs to the eukaryotic mitochondrial porin family and features a transmembrane beta-barrel structure.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-283), which encompasses the majority of the mature protein; this design may improve specificity for native VDAC3.
• For Western blot, a band around 31 kDa is expected; consider using mitochondrial-enriched lysates to enhance signal intensity.
• In IHC-P and IF/ICC, verify mitochondrial localization patterns; include appropriate controls to differentiate specific staining.
• For ELISA, the antibody can serve as a capture or detection reagent; optimize concentrations for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target procurement to diagnostic manufacturers and research institutes. This anti-VDAC3 polyclonal antibody is tailored for mitochondrial function and ion channel research, supporting assay development and biomarker discovery. With a comprehensive portfolio and technical expertise from concept to clinic, CamelBio ensures streamlined access to high-quality raw materials for your diagnostic needs.