Anti-VDAC3 (KD Validated) Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9Y277

Core Product Specifications and Parameters

Biological Background: VDAC3 Function and Localization

• VDAC3 (also known as outer mitochondrial membrane protein porin 3) is a non-selective voltage-gated ion channel that facilitates the transport of anions and cations across the mitochondrial outer membrane and plasma membrane (PubMed:31935282). Related references: PMID:31935282
• The channel demonstrates a stable high-conductance open state and voltage-dependent closure, with a mild preference for anions over cations (PubMed:31935282). Related references: PMID:31935282
• VDAC3 is essential for male fertility and contributes to the formation of the sperm mitochondrial sheath (By similarity).
• It is localized primarily to the mitochondrial outer membrane.
• The protein is widely expressed, with highest levels in testis (PubMed:9781040), and is also present in erythrocytes at the protein level (PubMed:27641616). Related references: PMID:9781040 PMID:27641616
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitination.
• Alternative splicing generates multiple isoforms.
• VDAC3 binds ATP and NAD, indicating nucleotide-binding functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human VDAC3; antibody specificity should be verified in the target species and application.
• For Western blot, the predicted band size is approximately 31 kDa; consider using total protein normalization or a mitochondrial loading control.
• In immunofluorescence and immunohistochemistry, co-staining with mitochondrial markers (e.g., Tom20, COX IV) can help confirm mitochondrial localization.
• Since the antibody is KD validated, it may be suitable for knockdown/knockout validation studies; however, optimal conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For mitochondrial and cell death research areas involving VDAC3, we provide bulk sourcing and rare target material procurement to support your assay development from concept to clinic.