Anti-VDAC2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P45880

Core Product Specifications and Parameters

Biological Background: VDAC2 Function and Localization

• VDAC2 (Voltage-Dependent Anion Channel 2), also known as outer mitochondrial membrane protein porin 2, is a non-selective voltage-gated ion channel that mediates transport of anions and cations across the mitochondrial outer membrane and plasma membrane. Related references: PMID:8420959
• The channel exhibits voltage-dependent gating, adopting an open conformation at 0 mV and transitioning to closed states at both positive and negative potentials, with two populations displaying distinct conductance and ion selectivity profiles. Related references: PMID:8420959
• VDAC2 binds various lipids, including ceramide, phosphatidylcholine, cholesterol, and oxysterol. Ceramide binding promotes mitochondrial outer membrane permeabilization (MOMP) and the apoptotic pathway. Related references: PMID:31015432
• It associates with the translocase of the outer mitochondrial membrane (TOM) complex and PINK1 kinase at depolarized mitochondria, stabilizing PINK1 and triggering mitophagy via recruitment of the E3 ubiquitin ligase PRKN. Related references: PMID:40080546
• VDAC2 also catalyzes phospholipid scrambling across the outer mitochondrial membrane independently of its channel activity, translocating both anionic and zwitterionic phospholipids.
• Localized to the mitochondrial outer membrane, VDAC2 is expressed in all tissues, with notable expression in erythrocytes. Related references: PMID:27641616 PMID:8420959
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitination, which may regulate channel function and mitochondrial processes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human VDAC2. For Western blot (WB), the predicted molecular weight is 32 kDa; verify band specificity with appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody concentration should be optimized for your specific tissue type and fixation method.
• Immunofluorescence (IF/ICC) may require permeabilization; consider testing in cell lines with abundant mitochondria.
• The antibody cross-reacts with human, mouse, and rat samples based on sequence homology; confirm reactivity in your experimental model.
• For ELISA, pair with a suitable detection antibody and validate performance against recombinant VDAC2 or native lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, technical services, and consulting—streamlining development from concept to clinic. Our portfolio includes validated antibodies for mitochondrial targets like VDAC2, optimized for assay integration. Beyond standard reagents, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a reliable supply chain for your diagnostic programs.