Anti-VDAC2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P45880

Core Product Specifications and Parameters

Biological Background: VDAC2 Function and Localization

• VDAC2 (Voltage-Dependent Anion Channel 2), also known as POR or outer mitochondrial membrane protein porin 2, is a non-selective voltage-gated ion channel that mediates the transport of anions and cations across the mitochondrial outer membrane and plasma membrane. Related references: PMID:8420959

• The channel exhibits two conductance states: an open high-conductance state and a voltage-dependent closed low-conductance state, contributing to metabolite exchange and mitochondrial function. Related references: PMID:8420959

• VDAC2 also catalyzes the scrambling of phospholipids across the outer mitochondrial membrane, translocating both anionic and zwitterionic phospholipids independent of its channel activity.

• It binds various lipids, including ceramide, which promotes mitochondrial outer membrane permeabilization (MOMP) and triggers the apoptotic pathway. Related references: PMID:31015432

• VDAC2 associates with the TOM complex and PINK1 kinase at depolarized mitochondria, stabilizing PINK1 and initiating mitophagy via Parkin recruitment. Related references: PMID:40080546

• The protein is localized to the mitochondrial outer membrane and is expressed in all tissues examined, with high expression in erythrocytes. Related references: PMID:27641616 PMID:8420959

• Post-translational modifications include acetylation, phosphorylation, ubiquitination, and isopeptide bond formation, reflecting its regulatory roles in cell signaling and metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human VDAC2; the monoclonal antibody recognizes a linear epitope within this sequence.
• For Western blot, the predicted molecular weight is approximately 32 kDa; consider using appropriate molecular weight markers and loading controls.
• In IHC-P, antigen retrieval may be necessary; validate the antibody in the relevant tissue type and species.
• The antibody cross-reacts with mouse and rat VDAC2 due to high sequence homology; confirm specificity in your model system.
• For ELISA, optimize coating antigen and antibody dilution to achieve the desired sensitivity and dynamic range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and optimized monoclonal and polyclonal antibodies. This anti-VDAC2 rabbit monoclonal antibody supports mitochondrial research, apoptosis, and mitophagy studies, and is part of our extensive catalog designed to accelerate your assay development. For rare or custom targets, our sourcing experts can help secure the critical raw materials you need from discovery through clinical application.