Anti-VDAC1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P21796

Core Product Specifications and Parameters

Biological Background: VDAC1 Function and Localization

• VDAC1 (Voltage-dependent anion-selective channel protein 1) is a non-selective voltage-gated ion channel encoded by the VDAC1 gene, also known as porin 1.
• It forms channels in the mitochondrial outer membrane and plasma membrane, mediating diffusion of anions and cations; involved in cell volume regulation and apoptosis. Related references: PMID:10661876 PMID:11845315 PMID:18755977
• The channel gates between open (weakly anion-selective) and closed (cation-selective) states depending on membrane potential. Related references: PMID:10661876 PMID:18755977 PMID:8420959
• VDAC1 binds signaling molecules like ceramide and cholesterol, influencing membrane properties and apoptosis. Related references: PMID:18755977 PMID:31015432
• It is a component of the permeability transition pore complex (PTPC), mediates cytochrome c release, and is regulated by PRKN/PINK1 to promote mitophagy or inhibit apoptosis. Related references: PMID:15033708 PMID:25296756 PMID:32047033 PMID:20230784
• VDAC1 also catalyzes phospholipid scrambling across the outer mitochondrial membrane, independent of its channel activity.
• Subcellular localization includes mitochondrial outer membrane, cell membrane, and membrane rafts.
• Expressed in heart, liver, skeletal muscle, and erythrocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider verifying the epitope specificity if precise mapping is needed.
• Validated applications include WB, IHC-P, IF/ICC, and ELISA. Optimal antibody dilutions and protocols should be determined by the end-user for each assay system.
• Due to VDAC1’s localization to the mitochondrial outer membrane, appropriate sample preparation (e.g., mitochondrial fractions or permeabilization) may enhance detection in IF/ICC and IHC-P.
• Cross-reactivity with human, mouse, and rat is reported; confirm reactivity in the relevant tissue or cell line.
• Shipping on ice packs maintains antibody stability; aliquot and store at -20°C or as recommended upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing support to streamline diagnostic development. The VDAC1 Rabbit mAb targets a key mitochondrial porin implicated in apoptosis, metabolism, and disease, fitting within portfolios focused on mitochondrial health and cell survival pathways. Whether you need this antibody in bulk or custom production of specialized reagents, CamelBio bridges your concept to clinical application.