Anti-VDAC1 / Porin Polyclonal Antibody for WB, IHC-P, ELISA - P21796

Core Product Specifications and Parameters

Biological Background: VDAC1 / Porin Function and Localization

• VDAC1 (Voltage-dependent anion-selective channel 1), also known as porin, is a 31 kDa multi-pass transmembrane protein belonging to the eukaryotic mitochondrial porin family.
• It functions as a non-selective voltage-gated ion channel, mediating anion and cation transport across the mitochondrial outer membrane and plasma membrane, and plays roles in cell volume regulation and apoptosis. Related references: PMID:10661876, PMID:11845315, PMID:18755977
• The channel adopts an open conformation at low membrane potential (weak anion selectivity) and a closed conformation at potentials above 30-40 mV (cation-selective). Related references: PMID:18755977, PMID:8420959
• VDAC1 is a key component of the permeability transition pore complex (PTPC), facilitating mitochondrial cytochrome c release and triggering apoptosis. Related references: PMID:15033708, PMID:25296756, PMID:20230784
• It participates in PRKN/PINK1-mediated mitophagy regulation; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination lowers mitochondrial calcium influx, inhibiting apoptosis. Related references: PMID:32047033
• VDAC1 binds various signaling molecules, including ceramide, phosphatidylcholine, cholesterol, and oxysterol. Related references: PMID:18755977, PMID:31015432
• Additionally, VDAC1 catalyzes phospholipid scrambling across the outer mitochondrial membrane, independent of its channel activity.
• The protein is predominantly expressed in heart, liver, skeletal muscle, and erythrocytes, with subcellular localization at the mitochondrial outer membrane, plasma membrane, and membrane rafts. Related references: PMID:27641616, PMID:8420959

Experimental Guidance and Technical Tips

• As a polyclonal antibody, consider including appropriate negative controls (e.g., pre-immune serum or knockout lysate) when performing Western blot or immunohistochemistry.
• For Western blot, a single band around 31 kDa is expected; pre-stain markers or positive control lysates from heart, liver, or skeletal muscle tissues can aid confirmation.
• In IHC-P, antigen retrieval methods (heat-induced or enzymatic) may enhance signal; validate staining in tissues with known high expression such as human heart or liver.
• This antibody has been validated for ELISA; end-users may explore sandwich ELISA pairing with a monoclonal capture antibody targeting a different VDAC1 epitope.
• Cross-reactivity with mouse and rat VDAC1 has been observed; validate detection in the relevant species and sample system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with seamless access to premium IVD raw materials. In the field of mitochondrial biology and apoptosis research, we offer validated antibodies like this VDAC1/Porin rabbit pAb, alongside custom monoclonal antibody development, bulk ancillary reagents, and rare-target sourcing to accelerate your assay pipeline from concept to clinic.