Anti-VDAC1 Polyclonal Antibody (KD Validated) for WB, ELISA - P21796

Core Product Specifications and Parameters

Biological Background: VDAC1 Function and Localization

• VDAC1 is a voltage-dependent anion-selective channel (porin) located primarily in the mitochondrial outer membrane, mediating the passive diffusion of small hydrophilic molecules and ions. Related references: PMID:10661876, PMID:11845315, PMID:18755977
• The channel adopts an open conformation at low membrane potential (weak anion selectivity) and closes at potentials above 30-40 mV, becoming cation-selective. Related references: PMID:10661876, PMID:18755977, PMID:8420959
• VDAC1 participates in apoptosis through cytochrome c release and by forming part of the permeability transition pore complex (PTPC). Related references: PMID:15033708, PMID:25296756, PMID:20230784
• It interacts with signaling molecules such as ceramide, phosphatidylcholine, and cholesterol, influencing cell volume regulation and apoptotic signaling. Related references: PMID:18755977, PMID:31015432
• Ubiquitination by PRKN modulates VDAC1 function: polyubiquitination promotes mitophagy, while monoubiquitination reduces calcium influx to inhibit apoptosis. Related references: PMID:32047033
• VDAC1 is also found at the cell membrane and membrane rafts, suggesting additional roles at the plasma membrane.
• Expressed in multiple tissues including erythrocytes, heart, liver, and skeletal muscle. Related references: PMID:27641616, PMID:8420959
• It catalyzes phospholipid scrambling across the outer mitochondrial membrane, a function separate from its channel activity.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human VDAC1 (aa 1-283), generating a polyclonal antibody expected to recognize multiple epitopes. This supports detection of endogenous VDAC1 across human, mouse, and rat samples.
• For Western blotting, the predicted molecular weight is ~31 kDa. Consider using mitochondrial enrichment or appropriate subcellular fractionation to enhance signal.
• KD validation confirms specific recognition of VDAC1; verify band specificity by knockdown or knockout controls in your experimental system.
• For ELISA, optimize antigen coating and antibody dilution based on your plate format and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a seamless supply chain for IVD raw materials, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For VDAC1-related research—spanning mitochondrial biology, apoptosis, and ion channel studies—we offer tailored sourcing solutions to meet your project needs. Our procurement network also supports rare-target reagent requests, ensuring you have the tools for every stage from concept to clinic.