Anti-VDAC 1/2/3 Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P21796 / P45880 / Q9Y277

Core Product Specifications and Parameters

Biological Background: VDAC 1/2/3 Function and Localization

• VDAC1 (Voltage-Dependent Anion Channel 1) is also known as porin 1, plasmalemmal porin, and outer mitochondrial membrane protein porin 1. It is a member of the VDAC family, which includes VDAC2 and VDAC3, all recognized by this antibody.
• It functions as a non-selective voltage-gated ion channel that mediates transport of anions and cations across the mitochondrial outer membrane and plasma membrane, playing critical roles in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977). Related references: PMID:10661876 PMID:11845315 PMID:18755977
• The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, with the open state being weakly anion-selective and the closed state cation-selective (PubMed:18755977, PubMed:8420959). Related references: PMID:18755977 PMID:8420959
• VDAC1 is localized to the mitochondrial outer membrane, cell membrane, and membrane rafts, consistent with its dual role in mitochondrial and plasma membrane transport.
• Post-translational modifications include acetylation and ubiquitination. Polyubiquitination by PRKN promotes mitophagy, while monoubiquitination decreases mitochondrial calcium influx to inhibit apoptosis (PubMed:32047033). Related references: PMID:32047033
• It binds various signaling molecules like ceramide, phosphatidylcholine, cholesterol, and oxysterol, modulating its activity and role in apoptosis (PubMed:18755977, PubMed:31015432). Related references: PMID:18755977 PMID:31015432
• Tissue expression is observed in erythrocytes, heart, liver, and skeletal muscle (PubMed:8420959, PubMed:27641616). Related references: PMID:8420959 PMID:27641616
• VDAC1 also catalyzes phospholipid scrambling across the outer mitochondrial membrane, independent of its channel activity, and is involved in mitochondrial permeability transition pore complex (PTPC) formation and cytochrome c release during apoptosis (PubMed:15033708, PubMed:25296756, PubMed:20230784). Related references: PMID:15033708 PMID:25296756 PMID:20230784

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal 1-100 amino acids of human VDAC1, a region conserved among VDAC1/2/3. Consider verifying sequence alignment if cross-reactivity to other species or isoforms is critical.
• For Western blotting, a band at ~31 kDa corresponding to VDAC1/2/3 is expected. Validate the antibody in your relevant cell or tissue lysate under optimal conditions.
• This antibody has been shown to work in immunofluorescence (IF-P), immunohistochemistry (IHC-P), and ELISA. Appropriate controls and antigen retrieval methods should be optimized for your specific samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering this anti-VDAC 1/2/3 antibody alongside a wide range of mitochondrial research tools. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, we provide expert raw-material sourcing to bridge the gap from concept to clinic.