Anti-VCP Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P55072

Core Product Specifications and Parameters

Biological Background: VCP Function and Localization

• Transitional endoplasmic reticulum ATPase (also designated p97 or VCP) that functions as a homohexameric chaperone and segregase in various cellular pathways.
• Central to the ubiquitin-proteasome system: the UFD1-VCP-NPLOC4 complex binds ubiquitinated misfolded proteins and exports them from the ER for cytosolic degradation.
• Critical for Golgi dynamics: necessary for mitotic fragmentation and post-mitotic reassembly of Golgi stacks, and facilitates vesicle budding from transitional ER.
• Recruited to DNA double-strand breaks in an RNF8/RNF168-dependent manner, promoting TP53BP1 accumulation and contributing to DNA repair. (PubMed:22020440, PubMed:22120668) Related references: PMID:22020440 PMID:22120668
• Essential for autophagy: required for maturation of ubiquitin-containing autophagosomes and mediates extraction of G3BP1 from stress granules to clear aggregates. (PubMed:20104022, PubMed:27753622, PubMed:38762759, PubMed:29804830, PubMed:34739333) Related references: PMID:20104022 PMID:27753622 PMID:38762759 PMID:29804830 PMID:34739333
• Negatively regulates type I interferon production by interacting with ubiquitinated RIGI, recruiting RNF125 to promote RIGI degradation. (PubMed:26471729) Related references: PMID:26471729
• Subcellular localization: Found in cytoplasm, cytosol, endoplasmic reticulum, nucleus, and stress granules, consistent with its multi-functional roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human VCP (P55072). This N-terminal region is expected to generate an antibody recognizing full-length VCP (~89 kDa) in denaturing western blots.
• For WB, use standard reducing conditions; the predicted molecular weight is 89 kDa, but post-translational modifications may cause slight shifts. Validate loading with appropriate controls.
• For IF/ICC, fixation and permeabilization conditions should be optimized; the antibody is expected to detect VCP in its reported locations (cytoplasm, ER, nucleus, stress granules).
• In ELISA, this antibody can be used for sensitive detection of recombinant or native VCP; consider pairing with a detection antibody for sandwich assays.
• Cross-reactivity with mouse and rat VCP has been demonstrated, making it suitable for studies across these species; nonetheless, perform a species-specific validation in your model system.
• As with any monoclonal antibody, lot-to-lot consistency is typical, but always titrate and validate in your specific application context using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. For your VCP (p97) research needs—whether in neurodegeneration, cancer, or cellular stress responses—CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials. Partner with us to streamline your assay development projects.