Anti-VCAM1 (CD106/INCAM-100) Polyclonal Antibody for WB, IF-P, ELISA - P19320

Core Product Specifications and Parameters

Biological Background: VCAM1 Function and Localization

• VCAM1 is an immunoglobulin superfamily cell adhesion molecule that exists in multiple isoforms due to alternative splicing, with the full-length form containing multiple immunoglobulin-like domains.
• VCAM1 mediates leukocyte adhesion to vascular endothelium by interacting with integrins, a key event in immune surveillance and inflammatory responses. Related references: PMID:31310649 PMID:10209034
• VCAM1 engagement during inflammation triggers calcium channel activation and RAC1 signaling, promoting leukocyte transendothelial migration. Related references: PMID:22970700
• In bone marrow, VCAM1 functions as a quality-control checkpoint for cellular entry by presenting a 'don't-eat-me' signal during MHC class-I presentation. Related references: PMID:35210567
• VCAM1 is primarily expressed on the cell surface and can be shed from the membrane as a secreted form.
• VCAM1 is highly expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cells in both normal and inflamed tissues.
• VCAM1 is a glycoprotein containing multiple immunoglobulin-like domains stabilized by disulfide bonds, and is subject to ubiquitin-like conjugation (Ubl).

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is ~81 kDa; however, due to glycosylation and potential shedding, multiple bands or a higher apparent mass may be observed. Validate under reducing and non-reducing conditions.
• For immunofluorescence (IF-P), consider testing both live-cell and fixed/permeabilized protocols, as VCAM1 is surface-exposed but also secreted. Endothelial cell lines stimulated with inflammatory cytokines (e.g., TNF-α) may enhance signal.
• For ELISA, this polyclonal antibody can be used for antigen capture or detection; optimize coating buffer and detection system for your specific assay format.
• Since this is a rabbit polyclonal antibody, lot-to-lot consistency may vary; we recommend pilot testing each new lot in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD manufacturers and researchers, providing validated antibody pairs, optimized polyclonal antibodies like this anti-VCAM1 antibody, and bulk ancillary reagents for immunology and inflammation research. Whether you need raw materials for leukocyte adhesion assays or rare-target sourcing, our one-stop service streamlines procurement from concept to clinic.