Anti-VCAM-1/CD106 Rabbit Polyclonal Antibody for WB, ELISA - P29533

Core Product Specifications and Parameters

Biological Background: VCAM-1/CD106 Function and Localization

• Vascular cell adhesion protein 1 (CD106/VCAM-1) is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, playing a critical role in immune surveillance and inflammation.
• It serves as a major regulator of leukocyte adhesion to the endothelium by interacting with integrins, facilitating transendothelial migration during inflammatory responses.
• Upon binding to its ligands on activated endothelial cells, CD106 triggers calcium channel activation and the small GTPase RAC1, promoting leukocyte extravasation.
• In the bone marrow, VCAM-1 acts as a quality-control checkpoint for entry, providing a 'don't-eat-me' signal in the context of major histocompatibility complex (MHC) class-I presentation.
• The protein is localized to the cell membrane and can also be secreted; it contains immunoglobulin domains, disulfide bonds, and is glycosylated.
• It is expressed on inflamed vascular endothelium, macrophage-like cells, and dendritic cells in both normal and inflamed tissues, with notable expression in the bone marrow.
• Alternative splicing generates multiple isoforms, and the protein is subject to ubiquitin-like conjugation (Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–310 of mouse CD106, corresponding to the extracellular domain; thus, the antibody may recognize both membrane-bound and secreted forms.
• For Western blot (WB), a band around 81 kDa is expected; consider using reducing conditions and validating in mouse endothelial cell lines or inflamed tissue lysates where VCAM-1 is upregulated.
• For ELISA applications, the antibody may be suitable for capture or detection of mouse VCAM-1; optimize assay conditions with recombinant protein or relevant biological samples.
• Cross-reactivity is reported for mouse; verify in your specific experimental system to confirm specificity and rule out off-target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge, providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials—from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance in sourcing rare-target raw materials for immunology and inflammation research, our streamlined supply solutions support your development of assays targeting molecules such as CD106/VCAM-1. Contact us to discuss how we can accelerate your next project.