Anti-VBP1 Polyclonal Antibody for WB, ELISA - P61758

Core Product Specifications and Parameters

Biological Background: VBP1 / Prefoldin Subunit 3 Function and Localization

• VBP1 encodes Prefoldin subunit 3, also known as von Hippel-Lindau-binding protein 1 (VBP-1) or PFDN3, and is a member of the prefoldin chaperone family.
• It specifically binds to the cytosolic chaperonin (c-CPN) and transfers target proteins to it, facilitating proper folding in the crowded cellular environment.
• VBP1 binds to nascent polypeptide chains, preventing aggregation and promoting correct folding pathways in competition with other non-native protein interactions.
• The protein localizes to both the cytoplasm and nucleus, consistent with its chaperone activity and potential regulatory roles.
• Expression is ubiquitous across human tissues, indicating a fundamental housekeeping function.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing to generate multiple isoforms.
• Structural studies have resolved the 3D conformation of VBP1, shedding light on prefoldin complex assembly and function.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human VBP1 protein (aa 1–197), making this polyclonal antibody suitable for detecting the endogenous protein in WB and ELISA applications.
• For Western blotting, consider testing both reducing and non-reducing conditions, as well as a range of lysate loadings, to confirm the band pattern at ~23 kDa in human and mouse samples.
• Validate antibody specificity in knockdown or overexpression systems, and include appropriate controls such as recombinant VBP1 protein or known positive cell lines.
• In ELISA formats, pre‑coat with recombinant antigen or capture the antibody, and optimise blocking and washing steps to minimise background.
• Cross‑reactivity with mouse VBP1 has been confirmed by the supplier; if working with other species, limited sequence homology may require verification before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with a streamlined supply chain for raw materials targeting chaperone biology and protein folding pathways. Our portfolio includes validated antibody pairs, optimised monoclonal and polyclonal antibodies, and bulk ancillary reagents—all supported by technical guidance from concept to clinic. For specialised targets such as prefoldin subunits, we offer custom sourcing and scalable production to help you accelerate diagnostic development.