Anti-VAV3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UKW4

Core Product Specifications and Parameters

Biological Background: VAV3 Function and Localization

• VAV3 is a guanine nucleotide exchange factor (GEF) that activates Rho family GTPases, including RhoA, RhoG, and to a lesser extent Rac1, by binding to their nucleotide-free states.
• It plays a critical role in angiogenesis; recruitment by phosphorylated EPHA2 is essential for EFNA1-induced RAC1 activation and vascular endothelial cell migration and assembly.
• In osteoclasts, VAV3 cooperates with SYK tyrosine kinase to mediate signaling through integrin αvβ1 (ITAGV-ITGB1), a pathway essential for proper cytoskeleton organization and osteoclast function, acting via RAC1 but not RHO.
• VAV3 is required for proper wound healing, specifically for phagocytic cup formation during macrophage phagocytosis of apoptotic neutrophils.
• It contributes to integrin β2 (ITGB2)-mediated macrophage adhesion, with a lesser role in β3 (ITGB3)-mediated adhesion, but does not affect β1 (ITGB1)-mediated adhesion.
• Isoform 1 and isoform 3 are widely expressed, with isoform 1 more abundant in adrenal gland, bone marrow, spleen, fetal brain, and spinal cord; isoform 3 is detected at very low levels in these tissues.
• The protein contains a Dbl homology (DH) domain for GEF activity, a pleckstrin homology (PH) domain, zinc finger domains, SH2 and SH3 domains, and is regulated by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 700–800 of human VAV3 (Q9UKW4); the antibody is recommended for Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA, as indicated by the supplier.
• Cross-reactivity has been confirmed for human and mouse VAV3; validation in other species should be performed by the end user.
• With a predicted molecular weight of 98 kDa, appropriate SDS-PAGE gel percentages and transfer conditions should be optimized to achieve clear band resolution.
• For IHC-P, antigen retrieval conditions and antibody dilution may require empirical optimization in the target tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our expertise spans rare target sourcing, enabling researchers and diagnostic manufacturers to access critical reagents like this anti-VAV3 antibody for studies in angiogenesis, oncology, and immunology. From concept to clinic, CamelBio provides reliable supply solutions tailored to your project needs.