Anti-VAV3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UKW4

Core Product Specifications and Parameters

Biological Background: VAV3 Function and Localization

• VAV3 is a guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP on Rho family GTPases, primarily RhoA, RhoG, and, to a lesser extent, Rac1, thereby regulating cytoskeletal dynamics.
• The protein exists as multiple isoforms; isoform 1 and isoform 3 are widely expressed, with isoform 3 being less abundant in certain tissues such as adrenal gland, bone marrow, and spleen.
• VAV3 plays a critical role in angiogenesis by mediating EPHA2-dependent RAC1 activation downstream of EFNA1, which promotes vascular endothelial cell migration and assembly.
• In osteoclasts, VAV3 cooperates with SYK tyrosine kinase to transduce signals from integrin alpha-v/beta-1 (ITGAV-ITGB1), activating RAC1 independently of RHO, which is essential for proper cytoskeleton organization and bone resorption.
• During wound healing, VAV3 is required for the formation of phagocytic cups in macrophages, enabling efficient phagocytosis of apoptotic neutrophils and proper tissue repair.
• The antibody targets a region within amino acids 560–680 of human VAV3, which encompasses conserved SH2 and SH3 domains, ensuring specificity for functional studies.

Experimental Guidance and Technical Tips

• Based on the immunogen design (C-terminal region a.a. 560–680), the antibody is expected to recognize both major isoforms of VAV3; consider verifying isoform specificity in your sample system.
• For Western blotting, the predicted molecular weight is approximately 98 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization protocols to detect VAV3, which localizes to subcellular compartments involved in cytoskeletal remodeling.
• Validate cross-reactivity in human and mouse samples, as indicated; for other species, perform a pilot experiment due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-VAV3 polyclonal antibody. We provide one-stop access to optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your projects from concept to clinical application. Let us bridge your VAV3-related research and assay development with reliable supply and technical expertise.