Anti-VAV2 Rabbit Polyclonal Antibody for WB, ELISA - P52735

Core Product Specifications and Parameters

Biological Background: VAV2 Function and Localization

• VAV2 is a guanine nucleotide exchange factor (GEF) for the Rho family of Ras-related GTPases, facilitating the exchange of GDP for GTP and thus activating downstream signaling cascades.
• It plays a pivotal role in angiogenesis, particularly in the context of EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
• VAV2 is recruited to the receptor tyrosine kinase EPHA2 upon phosphorylation, which is critical for coupling external signals to cytoskeletal reorganization via RAC1.
• The protein contains several conserved domains, including SH2 and SH3 domains, a Dbl homology (DH) domain for GEF activity, and a zinc-finger motif, indicative of its role as a phosphoprotein and metal-binding signaling scaffold.
• Alternative splicing of the VAV2 transcript results in multiple isoforms, which may fine-tune its regulatory functions in different cellular contexts.
• Widely expressed across tissues, VAV2 is implicated in fundamental processes such as cell migration, proliferation, and cytoskeletal dynamics.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 570–700 of human VAV2, a region likely encompassing part of the SH2 domain; consider this when comparing reactivity across species or splice variants.
• For Western blot, a band around 101 kDa is expected; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Validation in endothelial cells (e.g., HUVECs) or cell lines known to express EPHA2 may help confirm functional specificity in angiogenesis-related studies.
• When using ELISA, consider coating with the recombinant protein fragment or full-length protein to assess binding affinity and specificity.
• Cross-reactivity reported for human, mouse, and rat, but tissue-specific differences in expression levels should be verified with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, CROs, and research institutions with reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The VAV2 polyclonal antibody fits seamlessly into projects studying angiogenesis, GTPase signaling, and vascular biology, and CamelBio can assist in sourcing complementary rare-target reagents to accelerate your development pipeline.