Anti-VAV2 Rabbit Polyclonal Antibody for WB and ELISA - P52735

Core Product Specifications and Parameters

Biological Background: VAV2 Function and Localization

• Guanine nucleotide exchange factor (GEF) for Rho family GTPases, activating RAC1, CDC42, and RhoA by catalyzing GDP/GTP exchange.
• Critical mediator of angiogenesis; recruitment by phosphorylated EPHA2 is essential for EFNA1-induced RAC1 activation and vascular endothelial cell migration and assembly.
• Contains SH2 and SH3 domains, a zinc-finger DH domain, and a PH domain, characteristic of the VAV protein family, enabling protein-protein interactions and membrane targeting.
• Widely expressed across human tissues, supporting its broad regulatory roles in cell signaling.
• Undergoes tyrosine phosphorylation upon activation, which regulates its GEF activity and interaction with upstream receptors.
• Alternative splicing produces multiple isoforms, potentially diversifying its functional repertoire.
• Binds zinc and participates in metal-dependent signaling; classified as a phosphoprotein with roles in cytoskeletal rearrangement.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is 101 kDa; use a 6–10% SDS-PAGE gel and validate detection in lysates from endothelial or cancer cell lines known to express VAV2.
• The recombinant protein immunogen (aa 570-700) can serve as a positive control in ELISA assays; consider titration to determine optimal coating concentration.
• Polyclonal antibodies may recognize multiple epitopes, potentially increasing sensitivity but also background; optimize blocking and washing conditions accordingly.
• The [KO Validated] designation suggests the antibody is suitable for confirming VAV2 knockout samples; include wild-type and knockout cell lysates as controls.
• For cross-species studies, verify reactivity in mouse and rat tissues using appropriate secondary antibodies and loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports angiogenesis and GTPase signaling research, supplying high-quality reagents like this anti-VAV2 polyclonal antibody for applications from early development to clinical testing. We also specialize in sourcing rare-target raw materials, ensuring you have reliable access to critical components for diagnostic manufacturing.