Anti-VAV2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P52735

Core Product Specifications and Parameters

Biological Background: VAV2 Function and Localization

• VAV2 is a member of the VAV family of guanine nucleotide exchange factors (GEFs) for Rho family GTPases.
• It functions as a GEF, catalyzing the exchange of GDP for GTP on RAC1 upon recruitment by phosphorylated EPHA2, crucial for EFNA1-induced RAC1 activation.
• Plays an essential role in angiogenesis, promoting vascular endothelial cell migration and assembly (By similarity).
• The protein contains multiple conserved domains: an N-terminal calponin homology (CH) domain, a Dbl homology (DH) domain, a pleckstrin homology (PH) domain, an SH2 domain, and two SH3 domains, along with a C-terminal zinc finger.
• Widely expressed in various tissues, indicating its involvement in fundamental cellular processes.
• Alternative splicing generates multiple isoforms, and the protein undergoes phosphorylation, consistent with its regulatory role in signal transduction.
• Its 3D structure has been determined, providing molecular insights into GEF activity and domain interactions.
• The molecular weight of the canonical isoform is approximately 101 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human VAV2; the epitope lies within this region.
• For Western blotting, a band at approximately 101 kDa is expected; validate with appropriate positive and negative controls.
• In immunohistochemistry (paraffin-embedded), consider optimizing antigen retrieval and antibody dilution for your specific tissue type.
• ELISA conditions should be optimized for coating antigen and detection format; a standard indirect ELISA protocol may be a starting point.
• Cross-reactivity with mouse is indicated; however, verify suitability in your experimental system, especially when analyzing mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers this high-quality VAV2 rabbit monoclonal antibody as part of a comprehensive IVD raw material portfolio. Our one-stop sourcing platform supports researchers and diagnostic manufacturers with validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and custom rare-target sourcing. From angiogenesis to cell migration studies, count on CamelBio for reliable raw materials and technical consulting to accelerate your project from concept to clinic.