Anti-VASP Rabbit Polyclonal Antibody - P50552

Core Product Specifications and Parameters

Biological Background: VASP Function and Localization

• VASP (Vasodilator-stimulated phosphoprotein) is a member of the Ena/VASP family of actin-associated proteins.
• It promotes actin filament elongation by protecting barbed ends from capping and facilitating profilin-actin transfer.
• Plays critical roles in cytoskeleton remodeling-dependent processes such as axon guidance, cell migration, and lamellipodial dynamics.
• VASP regulates actin dynamics in platelets, contributing to platelet activation and aggregation.
• Subcellular localization includes cytoplasm, focal adhesions, tight junctions, lamellipodia, and filopodia membranes.
• Highly expressed in platelets.
• Post-translational modifications include phosphorylation; the protein is known to be an SH3-binding, coiled-coil containing phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human VASP. Confirm reactivity in target species (human, mouse, rat) via positive control lysates.
• For Western blot, expect a band around 40 kDa under reducing conditions. Validate specificity using VASP knockout or knockdown samples if available.
• For IHC-P and IF/ICC, optimize fixation (e.g., 4% PFA) and permeabilization conditions to preserve cytoskeletal structures. Include appropriate isotype controls.
• For ELISA, titrate the antibody and use a suitable coating antigen. Consider pairing with detection antibodies for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a reliable supply of IVD raw materials, including this anti-VASP polyclonal antibody for investigating actin dynamics and platelet biology. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Leverage our expertise to source rare-target raw materials and streamline your pipeline from concept to clinic.