Anti-VASP Rabbit Polyclonal Antibody for WB, ELISA - P50552

Core Product Specifications and Parameters

Biological Background: VASP Function and Localization

• Vasodilator-stimulated phosphoprotein (VASP) is an actin-associated protein belonging to the Ena/VASP family, critical for cytoskeleton remodeling and cell polarity.
• VASP promotes actin filament elongation by protecting barbed ends against capping and enhancing polymerization, especially in the presence of capping protein.
• It facilitates the transfer of profilin-bound actin monomers to growing filament barbed ends, accelerating elongation.
• VASP contributes to actin-based motility of pathogens such as Listeria monocytogenes in host cells.
• In platelets, VASP regulates actin dynamics and plays an important role in platelet aggregation.
• Subcellularly, VASP localizes to the cytoplasm, cytoskeleton, focal adhesions, tight junctions, and cell projections including lamellipodia and filopodia.
• Tissue specificity data indicate high expression in platelets.
• Post-translational modifications include acetylation and phosphorylation; VASP contains SH3-binding motifs and a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide within amino acids 150-250 of human VASP; verify its specificity in your target cell or tissue system.
• For Western blotting, a band near 40 kDa is expected; optimize antibody dilution based on endogenous expression levels in your sample.
• In ELISA applications, consider pairing with a validated detection antibody and calibrating against a recombinant VASP standard curve.
• Since VASP is highly expressed in platelets, platelet lysates are a suitable positive control; however, validate performance in other cell types (e.g., adherent cells) where expression may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, offering a streamlined pathway from concept to clinic. This anti-VASP antibody supports research into cytoskeletal dynamics and platelet biology, and our portfolio extends to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials. Whatever your procurement needs for diagnostic development, CamelBio is ready to bridge the supply gap.