Anti-VASP Rabbit Monoclonal Antibody for WB, ELISA - P50552

Core Product Specifications and Parameters

Biological Background: VASP Function and Localization

• Vasodilator-stimulated phosphoprotein (VASP) is an actin-associated protein encoded by the VASP gene in humans (Gene ID: 7408, UniProt: P50552).
• VASP belongs to the Ena/VASP family of proteins that regulate cytoskeleton remodeling and cell polarity processes, including axon guidance, lamellipodial/filopodial dynamics, and cell migration.
• It promotes actin filament elongation by protecting barbed ends from capping and increasing polymerization rates, partly through the transfer of profilin-bound actin monomers.
• VASP plays a critical role in actin-based motility of pathogens such as Listeria monocytogenes in host cells.
• In platelets, VASP regulates actin dynamics and is a key modulator of platelet aggregation; it is highly expressed in platelets.
• Subcellular localization includes the cytoplasm, cytoskeleton, focal adhesions, tight junctions, and membrane of cell projections like lamellipodia and filopodia.
• The protein undergoes phosphorylation and acetylation, and contains SH3-binding motifs, a coiled-coil domain, and EVH1/EVH2 domains for protein interactions (Keywords: Phosphoprotein, Acetylation, SH3-binding).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), suggesting the antibody may detect full-length VASP across potential isoforms; verify isoform specificity in your system.
• For Western blotting, expect a band near 40 kDa; use platelet lysate or other VASP-expressing cells as a positive control.
• For ELISA applications, consider coating with recombinant human VASP protein or peptide and optimize detection conditions.
• The antibody is validated for human samples; cross-reactivity with other species has not been determined and should be tested if required.
• Include appropriate controls and validate in your relevant sample types (e.g., cell lines, tissue homogenates) before drawing conclusions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and researchers, offering a wide range of validated antibodies like this anti-VASP monoclonal antibody for actin dynamics and platelet studies. Beyond individual reagents, we supply optimized antibody pairs, bulk ancillary reagents, and support custom sourcing of rare target raw materials to accelerate your assay development. Partner with us to secure high-quality, ready-to-use tools for your next diagnostic project.