Anti-VAPB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95292

Core Product Specifications and Parameters

Biological Background: VAPB Function and Localization

• VAPB (Vesicle-associated membrane protein-associated protein B/C, aliases ALS8, VAP-B, VAMP-B) is an ER-anchored membrane protein encoded by the VAPB gene in humans.
• Mediates contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 and WDR44. Related references: PMID:32344433, PMID:33124732
• Interaction with STARD3 requires phosphorylation of its FFAT motif. Related references: PMID:33124732
• Participates in export of neosynthesized proteins from the ER through WDR44 interaction. Related references: PMID:32344433
• Induces ERN1/IRE1 activity as part of the unfolded protein response (UPR). Related references: PMID:16891305, PMID:20940299
• Regulates cellular calcium homeostasis. Related references: PMID:22131369
• Subcellularly localized to the endoplasmic reticulum membrane.
• Ubiquitously expressed with isoform 1 predominating. Undergoes acetylation, phosphorylation, and ubiquitination; linked to amyotrophic lateral sclerosis and host-virus interactions.

Experimental Guidance and Technical Tips

• The recombinant immunogen corresponds to amino acids 10–222, covering the cytoplasmic region of VAPB; this design may detect both full-length and potential N-terminal fragments.
• For Western blot, anticipated band size is ~27 kDa; a reducing SDS-PAGE and standard transfer protocols are typically suitable.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions for ER membrane protein detection.
• Verify antibody specificity by comparing staining patterns with known VAPB subcellular localization or using knockout/knockdown controls.
• For ELISA, pair with a validated capture/detection antibody or use as a detection reagent after confirming reactivity against native or recombinant VAPB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with integrated sourcing of IVD raw materials, from validated antibody pairs to optimized monoclonal/polyclonal antibodies and ancillary reagents. For VAPB detection in ER contact site dynamics or unfolded protein response pathways, we offer tailored procurement solutions to accelerate your assay development. Contact us to discuss bulk and custom raw-material needs for neurodegeneration and cell biology research.