Anti-VAPA Polyclonal Antibody for WB, IHC-P, ELISA - Q9P0L0

Core Product Specifications and Parameters

Biological Background: VAPA Function and Localization

• VAPA (Vesicle-associated membrane protein-associated protein A), also designated VAP-33 or VAP-A, is encoded by the VAPA gene in humans.
• As an ER-resident protein, VAPA establishes membrane contact sites between the ER and endosomes via interactions with FFAT motif-containing proteins like STARD3 and WDR44, facilitating cholesterol transfer and nascent protein export. Related references: PMID:33124732, PMID:32344433
• VAPA can be recruited to the plasma membrane by OSBPL3, where the complex modulates RRAS signaling and attenuates integrin beta-1 activation, and has been implicated in ER morphology regulation and vesicle trafficking. Related references: PMID:25447204, PMID:16143324, PMID:11511104, PMID:19289470
• Subcellularly, VAPA localizes to the endoplasmic reticulum membrane, cell membrane, tight junctions, and the nuclear membrane.
• It is ubiquitously expressed across tissues.
• The protein features a coiled-coil domain, a transmembrane helix, and undergoes acetylation and phosphorylation; it is also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 137-227) of human VAPA, potentially allowing detection of multiple isoforms; validate in the relevant sample system.
• For Western blot, expect a band near 28 kDa; use appropriate positive and negative controls.
• In IHC-P, optimize antigen retrieval and antibody dilution for the tissue of interest.
• ELISA can be performed with recombinant VAPA as a standard; titration of coating antigen and antibody may be needed.
• The antibody cross-reacts with mouse VAPA; consider species-specific validation if using mouse-derived systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your single-source partner for IVD raw materials, offering validated antibody pairs, high-specificity polyclonal antibodies, and ancillary reagents to streamline your assay development. For studies on endoplasmic reticulum dynamics and vesicular trafficking, our anti-VAPA polyclonal antibody provides a reliable tool. Whether you need bulk quantities or custom sourcing of rare targets, CamelBio supports your program from concept to clinic.