Anti-VAMP8 Rabbit Polyclonal Antibody for WB, ELISA - Q9BV40

Core Product Specifications and Parameters

Biological Background: VAMP8 Function and Localization

• VAMP8 (Vesicle-associated membrane protein 8), also known as Endobrevin (EDB), is a v-SNARE protein that plays a critical role in intracellular membrane fusion events.
• It forms a SNARE complex with the t-SNAREs STX11 and SNAP23, which mediates the regulated exocytosis of cytotoxic granules in natural killer cells and cytotoxic T-lymphocytes (PubMed:28265073). Related references: PMID:28265073
• VAMP8 is involved in autophagy by mediating the fusion of autophagosomes with lysosomes through a SNARE complex containing SNAP29 and STX17 (PubMed:23217709, PubMed:25686604). Related references: PMID:23217709 | PMID:25686604
• Required for dense-granule secretion in platelets, VAMP8 plays a key role in hemostasis (PubMed:12130530). Related references: PMID:12130530
• It also participates in the activation of the type I interferon antiviral response via a TRIM6-dependent mechanism (PubMed:31694946). Related references: PMID:31694946
• VAMP8 localizes to the lysosome, early and late endosomes, cell membrane, and zymogen granule membranes, reflecting its diverse roles in vesicular trafficking.
• Post-translational modifications include acetylation and phosphorylation, and it is annotated with keywords such as antiviral defense, autophagy, and protein transport.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant fusion protein covering amino acids 1-75 of human VAMP8, corresponding to the N-terminal cytoplasmic domain. It is expected to recognize both human and cross-reactive species (mouse, rat) due to sequence conservation.
• For Western blotting, a band of approximately 11 kDa is anticipated; use a high-percentage gel (e.g., 12-15%) and reducing conditions. Validate with positive control lysates from human, mouse, or rat cells known to express VAMP8 (e.g., immune cells, platelets).
• In ELISA applications, the antibody can be used as a capture or detection reagent. Pairing with a monoclonal anti-VAMP8 antibody may improve specificity in sandwich ELISA formats. Optimize coating concentrations empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this VAMP8 rabbit pAb, and bulk ancillary reagents to support diagnostic development in immune function, autophagy, and hemostasis research areas. We also provide rare target raw-material sourcing and technical consulting to help streamline your assay development from concept to clinic.