Anti-VAMP4 Rabbit Polyclonal Antibody for WB and ELISA - O75379

Core Product Specifications and Parameters

Biological Background: VAMP4 Function and Localization

• VAMP4 (Vesicle-associated membrane protein 4), also known as VAMP-4 or VAMP24, is a v-SNARE protein that mediates vesicle docking and fusion with target membranes.
• It forms a component of the core SNARE complex, which consists of a parallel four-helix bundle involving three t-SNARE helices and one v-SNARE helix, driving membrane fusion (PubMed:10036234).
  Related references:
  PMID:10036234
• Belongs to the synaptobrevin family of SNARE proteins, characterized by a conserved SNARE motif and a C-terminal transmembrane domain for membrane anchoring.
• Localizes to the cytoplasmic vesicle membrane, consistent with its role in trafficking between intracellular compartments and the plasma membrane.
• By similarity, in skeletal muscle satellite cells, VAMP4 cooperates with STX11 and SNAP23 to facilitate CD36 transport from endosomes to the membrane, crucial for muscle regeneration.
• Post-translational modifications include phosphorylation, and the protein is identified by proteomics; it undergoes alternative splicing producing isoforms.
• Keywords associated with VAMP4 include: Coiled coil, Transmembrane, Transmembrane helix, Cytoplasmic vesicle, Phosphoprotein, Alternative splicing, and Reference proteome.

Experimental Guidance and Technical Tips

• For Western blot, since the predicted molecular weight is 16 kDa, use a high-percentage resolving gel (e.g., 12–15% SDS-PAGE) to achieve optimal separation and transfer.
• The immunogen corresponds to the N-terminal cytoplasmic domain (aa 1–115) of human VAMP4; this region shows high conservation across human, mouse, and rat, supporting the reported cross-reactivity.
• In ELISA, consider coating with the recombinant immunogen fragment for capture or indirect detection; validate antibody specificity under your assay conditions.
• As a polyclonal antibody, lot-to-lot variability may occur; include positive and negative controls and consider titration to determine optimal working dilution for your specific application.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline diagnostic and research workflows by providing a comprehensive portfolio of IVD raw materials, from validated antibodies to bulk ancillary reagents. Whether you are investigating SNARE-mediated membrane trafficking or developing assays targeting VAMP4, our sourcing platform delivers high-quality antibodies and tailored support to meet your project needs. Partner with CamelBio to accelerate your biomarker discovery and assay development.