Anti-VAMP3/Cellubrevin Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q15836

Core Product Specifications and Parameters

Biological Background: VAMP3/Cellubrevin Function and Localization

• Vesicle-associated membrane protein 3 (VAMP3), also known as cellubrevin (CEB) and synaptobrevin-3, is a member of the SNARE protein family.
• VAMP3 functions as a SNARE protein mediating vesicular transport from late endosomes to the trans-Golgi network.
• It is localized to early endosome membranes, recycling endosome membranes, and is also found in synapses and synaptosomes.
• VAMP3 possesses a coiled-coil domain and a transmembrane helix, characteristic of SNARE proteins involved in membrane fusion.
• Post-translational modifications include acetylation, isopeptide bond formation, and ubiquitin conjugation (Ubl conjugation), which may regulate its activity or localization.
• Keywords from UniProt highlight its roles in protein transport, endosome function, and synaptic processes.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1-100) of human VAMP3. This region may be suitable for detecting both full-length and possibly truncated forms, but validation in your specific sample system is recommended.
• For western blot (WB) analysis, the predicted molecular weight of VAMP3 is approximately 11 kDa, though observed bands may be higher due to post-translational modifications or dimerization. Use appropriate molecular weight markers and consider loading appropriate controls.
• In immunofluorescence (IF/ICC) studies, VAMP3 localizes to early and recycling endosomes as well as synaptic structures. Adequate permeabilization and fixation are critical for optimal detection of membrane-associated targets.
• For ELISA applications, ensure that the antigen coating and detection conditions are optimized for recombinant or native VAMP3 proteins.
• Cross-reactivity is reported for human; when using in other species, sequence homology should be examined, and empirical validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-VAMP3 antibody for studies in membrane trafficking. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to streamline your development pipeline.