Anti-VAMP3/Cellubrevin Polyclonal Antibody for WB and ELISA - Q15836

Core Product Specifications and Parameters

Biological Background: VAMP3/Cellubrevin Function and Localization

• VAMP3, also known as Cellubrevin or Synaptobrevin-3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family of SNARE proteins.
• It functions as a SNARE protein that mediates the fusion of vesicles with target membranes, specifically facilitating vesicular transport from late endosomes to the trans-Golgi network.
• VAMP3 is predominantly localized to the membranes of early and recycling endosomes, and is also found in synaptic vesicles and synaptosomes.
• Post-translational modifications include acetylation and ubiquitin-like conjugation (e.g., ISGylation), which may regulate its trafficking or stability.
• This protein has been identified in proteomics studies and its sequence directly confirmed by protein sequencing.
• Its role in endosomal recycling and membrane trafficking makes it a key player in processes such as receptor recycling, cell migration, and neurotransmitter release.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein covering the N-terminal region (aa 1-80) of human VAMP3. It is expected to recognize the full-length protein in denaturing and native conditions.
• For Western blot (WB), a band of approximately 11 kDa is anticipated. Optimize the primary antibody dilution in the 1:500–1:2000 range and validate in your specific sample system.
• The antibody cross-reacts with mouse and rat VAMP3 based on supplier data; however, it is advisable to verify species reactivity in your experimental setup.
• For ELISA applications, the recombinant immunogen may be used as a positive control to establish a standard curve.
• Store aliquots at -20°C for long-term stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating vesicular trafficking, endosomal dynamics, or synaptic function, this VAMP3 antibody is part of our extensive portfolio of SNARE protein reagents. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and offer rare target raw-material sourcing to streamline your assay development and scale-up needs.