Anti-VAMP2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P63027

Core Product Specifications and Parameters

Biological Background: VAMP2 Function and Localization

• VAMP2, also known as synaptobrevin-2, is a vesicle-associated membrane protein that functions as a v-SNARE in intracellular membrane fusion events.
• It assembles into a core SNARE complex with t-SNAREs SNAP25 and STX1A to mediate the fusion of synaptic vesicles with the presynaptic plasma membrane, crucial for neurotransmitter release.
  Related references: PMID:10036234, PMID:30929742
• Beyond its role in exocytosis, VAMP2 is involved in compensatory endocytosis for synaptic vesicle recycling and, in adipocytes, insulin-dependent exocytosis of GLUT4 storage vesicles.
• VAMP2 may independently modulate the gating characteristics of the voltage-dependent potassium channel KCNB1.
• It is primarily localized to synaptic vesicle membranes and the cell membrane, consistent with its role in vesicle trafficking.
• Tissue expression is predominantly in the nervous system and skeletal muscle.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and mutations are associated with intellectual disability.
• Keywords: SNARE, exocytosis, neurotransmitter transport, synapse, membrane fusion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human VAMP2; consider this when detecting isoforms or cleavage products.
• For Western blotting, a band at approximately 13 kDa is expected; optimize gel percentage and transfer conditions for low-molecular-weight proteins.
• In immunofluorescence and immunohistochemistry, validate antibody specificity in relevant neuronal tissues or cell lines known to express VAMP2.
• For ELISA, the immunogen peptide may serve as a suitable positive control to assess assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to high-quality IVD raw materials. For neuroscience and SNARE-related research, we offer a range of monoclonal antibodies, including this Anti-VAMP2 antibody, along with complementary reagents and technical support. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio is your sourcing partner from concept to clinic.