Anti-VAMP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P23763

Core Product Specifications and Parameters

Biological Background: VAMP1 Function and Localization

• VAMP1, also known as synaptobrevin-1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family.
• It plays a critical role in the targeting and/or fusion of transport vesicles to their target membrane, particularly in neurotransmitter release.
• The protein localizes to synaptic vesicle membranes, cytoplasmic vesicle membranes, and also to the mitochondrial outer membrane, suggesting diverse trafficking functions.
• Expression is predominantly observed in the nervous system, skeletal muscle, and adipose tissue, reflecting its importance in excitable and metabolic tissues.
• VAMP1 undergoes alternative splicing, producing isoforms that may differ in function or localization.
• Mutations in VAMP1 are associated with congenital myasthenic syndrome (CMS), a neuromuscular disorder, underscoring its role in synaptic transmission.
• Post-translational modifications include phosphorylation, and the protein contains a transmembrane helix and coiled-coil domains that mediate protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–96) of human VAMP1, which may generate antibodies recognizing multiple isoforms.
• For Western blot, a band around 13 kDa is expected; consider using reducing conditions and appropriate membrane blocking.
• This antibody has been reported to detect VAMP1 in human, mouse, and rat samples; validate in your specific sample system.
• IHC-P and ELISA applications are supported; optimize antigen retrieval and antibody dilution empirically.
• Since VAMP1 is a membrane protein, consider using detergents in lysis buffers to ensure solubility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neuroscience and membrane trafficking research, CamelBio offers validated antibody pairs, optimized polyclonal antibodies like this VAMP1 antibody, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your diagnostic and research development pipeline.